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TrypanoFAN - Standard Operating Procedures




Download All Protocols
(pdf)

Note - this is a new version of TrypanoFAN powered by Django. The text in the protocols document referring to the backend/data input is not valid here. Instead, a web interface for data entry/edits is available. Please contact mcf34@cam.ac.uk for details.

These protocols are designed for high-throughput (~500 genes by 12/04) and represent the strict methodology used within the project. Comments welcome.

RNAi experiments are performed on the Single Marker Bloodstream (SMB) cell-line described by Wirtz et al, 1999 Mol. Biochem. Parasitol. 99: 89-101. Fig. 7.

Principal reagents and kits used were sourced from the following companies: